Effectiveness Test of Soursop Leaf Ethanol Extract (Annona muricata L) as a Healer of Incision Wounds On Mice (Mus musculus) Skin

Abstract

Soursop leaves (Annona muricata L.) are one of the traditional plants that are useful for healing wounds. Wounds are a condition that is often experienced by everyone, whether with mild, moderate or severe severity. Wounds are damage to part of the body's tissue. This study aims to determine the effectiveness of soursop leaf ethanol extract (Annona muricata L.) as a healer of incision wounds on mice (Mus musculus) skin. The method used in making the extract is the maceration process of simplicia using 96% ethanol. The test animals consisted of 15 white mice (Mus musculus), which were divided into 5 treatments. The first treatment was negative control (aquadest), the second treatment was positive control (povidone iodine 10%), the third treatment was extract 0.1 grams, the fourth treatment was 0.2 grams and the fifth treatment was 0.3 grams. In each treatment, an incision was made on the back of the test animal. The treatment was given once a day for 14 days. The results of this study indicate that ethanol extract of soursop leaves (Annona muricata L.) can accelerate the healing of incision wounds in test animals, mice (Mus musculus), namely with the treatment of 0.3 grams of extract. The results of the anova statistical test on incision wounds in mice using soursop leaf extract obtained a significance value of P> 0.05 (at a level of 0.05, namely 0.000 <0.005), meaning that each treatment gave a meaningful difference (significant), for this it is necessary to continue with testing the average difference of each treatment with the Post hoc tests LSD test.